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pDNA Production and Purification

In recent years, there has been a growing demand for DNA-based therapeutics, commonly known as gene therapy, to combat genetically-based pathologies. Because plasmid DNA have significantly different biophysical characteristics than proteins, their production can have a drastic impact on downstream purification unit operations. This educational webcast explores the challenges faced by pDNA manufacturers and how Lonza's pDNA Production Platform addresses those challenges. More.

Improving IEX Throughput and Performance with Differentiated Chromatography Sorbents

As upstream processing becomes more optimized, it increases the amount of impurity and contaminants to be removed from large volumes of feedstock. With this added pressure on downstream processing, there is a pressing need for more efficient chromatography operations. In this paper, the authors describe how they characterized differentiated selectivity of Q and S HyperCel ion-exchange sorbents. Click here to learn more about their methods and the results of their testing in this informative paper.

Single-Use, Continuous-Countercurrent, Multicolumn Chromatography

To address the challenge of increased downstream bottlenecks, some manufacturers are turning to multicolumn chromatography. The principles of simulated moving-bed technology can be applied to create a continuous-countercurrent process. The first column in a loading train is allowed to break through, and material coming off it is captured on a second column. Binding capacity can thus be exploited beyond dynamic binding capacity. These processes often significantly improve media use — and hence significantly reduce consumption of media and buffers. More.

A Presanitized, Purpose-Designed, Single-Use TFF Strategy

Sius “single-use” cassettes provide a unique solution for a cost-effective downstream processing, with measurable cost-in-use savings, while eliminating a significant amount of time performing burdensome clean-in-place (CIP) procedures. This case study outlines the experimental design, development studies, and proposed single-use commercial TFF process based on work performed by Mike LaBreck at Groupe NovaSep. More.